Hotspot

psbZ-trnS-UGA

LSC · 25237-25571 · 335 bp

Back to Lespedeza

Pi 0.00813

MarkerSeek score 42.80

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: LSC
length_bp: 335
variable_sites: 9
pi: 0.00813
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	ACTTGACCAACCTTCAGGAG	CAACCACTCAGCCATCTCTC	50.0	55.0	57.1	58.0	457-462 bp	1.0	74.7
psbZ-trnS-UGA_p2	ACTTGACCAACCTTCAGGAG	TTCGCCGAGAAAAAGGAGAG	50.0	50.0	57.1	57.9	542-547 bp	1.0	73.9
psbZ-trnS-UGA_p3	ACTTGACCAACCTTCAGGAG	GTTTTCAAGACCGGGGCTAT	50.0	50.0	57.1	57.9	477-482 bp	1.0	73.8
psbZ-trnS-UGA_p4	GGAGAAGCAAATACAACGGG	CAACCACTCAGCCATCTCTC	50.0	55.0	56.8	58.0	441-446 bp	1.0	72.7
psbZ-trnS-UGA_p5	CTTGACCAACCTTCAGGAGA	CAACCACTCAGCCATCTCTC	50.0	55.0	56.8	58.0	456-461 bp	1.0	72.4