Hotspot

ycf3-psaA

LSC · 18433-19113 · 681 bp

Back to Lespedeza

Pi 0.0082

MarkerSeek score 54.90

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 681
variable_sites: 23
pi: 0.0082
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AAGTTGCGGAGTCTTGGTTT	TTTCACTTCTGGTTCCGGTG	45.0	50.0	57.9	58.0	831-842 bp	1.0	82.2
ycf3-psaA_p2	TTGGTTGAAGATCACTGGGC	TTTCACTTCTGGTTCCGGTG	50.0	50.0	58.1	58.0	743-754 bp	1.0	82.2
ycf3-psaA_p3	TGGTTTAATCAAGCCGCTGA	TTTCACTTCTGGTTCCGGTG	45.0	50.0	57.8	58.0	817-828 bp	1.0	81.1
ycf3-psaA_p4	AGATCACTGGGCGTTTTGAA	TTTCACTTCTGGTTCCGGTG	45.0	50.0	57.7	58.0	735-746 bp	1.0	80.8
ycf3-psaA_p5	AAGCTATAGCCCTTACCCCC	TTTCACTTCTGGTTCCGGTG	55.0	50.0	58.3	58.0	786-797 bp	1.0	80.7