Hotspot

trnL-UAA-trnT-UGU

LSC · 14049-14939 · 891 bp

Back to Lespedeza

Pi 0.0068

MarkerSeek score 51.30

Primer available yes

Label trnL-UAA-trnT-UGU

Feature Metadata

label_name: trnL-UAA-trnT-UGU
feature_type: igs
region: LSC
length_bp: 891
variable_sites: 20
pi: 0.0068
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	GCGTCTACCTATTTCGCCAT	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.8	57.9	947-992 bp	1.0	80.6
trnL-UAA-trnT-UGU_p2	CCCATTGTGAATTCCAGGGT	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.8	57.9	1034-1079 bp	1.0	79.7
trnL-UAA-trnT-UGU_p3	GCGTCTACCTATTTCGCCAT	GGATTCGAACCGATGACCAT	50.0	50.0	57.8	57.8	979-1024 bp	1.0	79.7
trnL-UAA-trnT-UGU_p4	CCCATTGTGAATTCCAGGGT	GGATTCGAACCGATGACCAT	50.0	50.0	57.8	57.8	1066-1111 bp	1.0	78.8
trnL-UAA-trnT-UGU_p5	TGCCCATTGTGAATTCCAGG	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.4	57.9	1036-1081 bp	1.0	78.1