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rps19

LSC · 83016-83294 · 279 bp

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Pi 0.00717

MarkerSeek score 43.20

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 2
pi: 0.00717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TGCTGCTGCAGAATAAACCA	GATGAAGGAACGGGAAGACC	45.0	55.0	58.1	58.0	1257-1269 bp	1.0	80.6
rps19_p2	TGCTGCTGCAGAATAAACCA	CCAGATGAAGGAACGGGAAG	45.0	55.0	58.1	58.0	1260-1272 bp	1.0	80.6
rps19_p3	GCAATTCCCTCAACACCAGA	AGTAGGGAATGTTGGGGTGA	50.0	50.0	58.1	57.9	1324-1336 bp	1.0	80.6
rps19_p4	GCAATTCCCTCAACACCAGA	GATGAAGGAACGGGAAGACC	50.0	55.0	58.1	58.0	1734-1746 bp	1.0	80.5
rps19_p5	GCAATTCCCTCAACACCAGA	CCAGATGAAGGAACGGGAAG	50.0	55.0	58.1	58.0	1737-1749 bp	1.0	80.4