Hotspot

ndhA

SSC · 119936-122136 · 2201 bp

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Pi 0.00849

MarkerSeek score 65.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2201
variable_sites: 18
pi: 0.00849
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCCGGTTACCATAGGAAGCA	GCCCACCCGGTTTTATCAAT	50.0	50.0	58.1	58.5	2333-2413 bp	1.0	78.1
ndhA_p2	TCCGGTTACCATAGGAAGCA	GCCCACCCGGTTTTATCAA	50.0	52.6	58.1	58.0	2333-2413 bp	1.0	74.0
ndhA_p3	CCGGTTACCATAGGAAGCAT	GCCCACCCGGTTTTATCAAT	50.0	50.0	57.1	58.5	2332-2412 bp	1.0	71.7
ndhA_p4	TCCGGTTACCATAGGAAGCA	TGGGAGAAGTTGATCGTTGA	50.0	45.0	58.1	56.5	2234-2314 bp	1.0	70.3
ndhA_p5	ATCCGGTTACCATAGGAAGC	GCCCACCCGGTTTTATCAAT	50.0	50.0	56.8	58.5	2334-2414 bp	1.0	69.4