Hotspot

ndhG-ndhI

SSC · 119103-119342 · 240 bp

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Pi 0.0167

MarkerSeek score 62.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 240
variable_sites: 4
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATTGGTCCAGGCAAATCCAT	GTCGTTTACCACTGTCAGTA	45.0	45.0	57.4	54.8	354-355 bp	1.0	51.0
ndhG-ndhI_p2	ATTGGTCCAGGCAAATCCAT	GTCGTTTACCACTGTCAGTAA	45.0	42.9	57.4	55.6	354-355 bp	1.0	49.5
ndhG-ndhI_p3	TCATGTATTGGTCCAGGCAA	GTCGTTTACCACTGTCAGTA	45.0	45.0	56.8	54.8	360-361 bp	1.0	45.9
ndhG-ndhI_p4	ATTGGTCCAGGCAAATCCAT	GTCGTTTACCACTGTCAGTAAT	45.0	40.9	57.4	56.1	354-355 bp	1.0	45.8
ndhG-ndhI_p5	TATTGGTCCAGGCAAATCCA	GTCGTTTACCACTGTCAGTA	45.0	45.0	56.8	54.8	355-356 bp	1.0	45.8