Hotspot

rps19-rpl2

IRb · 83295-83351 · 57 bp

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Pi 0.13

MarkerSeek score 83.40

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 57
variable_sites: 6
pi: 0.13
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	CCCGGGACCACGAAATTATT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.0	58.1	182-193 bp	1.0	85.4
rps19-rpl2_p2	CCCGGGACCACGAAATTATT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	274-285 bp	1.0	84.1
rps19-rpl2_p3	CCCGGGACCACGAAATTATT	ATTTGATTCTTCGTCGCCGT	50.0	45.0	58.0	58.3	184-195 bp	1.0	83.9
rps19-rpl2_p4	CCCGGGACCACGAAATTATT	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.0	57.8	240-251 bp	1.0	83.4
rps19-rpl2_p5	CCCGGGACCACGAAATTATT	TTTGATTCTTCGTCGCCGTA	50.0	45.0	58.0	57.6	183-194 bp	1.0	83.4