Hotspot

petG-trnW-CCA

LSC · 64857-64985 · 129 bp

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Pi 0.0236

MarkerSeek score 66.00

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 3
pi: 0.0236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	TGGCGATCAATTGGACCTTT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	57.8	58.0	202-204 bp	1.0	80.0
petG-trnW-CCA_p2	TGGCGATCAATTGGACCTTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	57.8	58.0	224-226 bp	1.0	79.9
petG-trnW-CCA_p3	ACTTTGGCCGGATTATTCGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	57.6	58.0	268-270 bp	1.0	78.1
petG-trnW-CCA_p4	ACTTTGGCCGGATTATTCGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	57.6	58.0	246-248 bp	1.0	78.0
petG-trnW-CCA_p5	GTGGCGATCAATTGGACCTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.5	58.0	225-227 bp	1.0	77.4