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petA-psbJ

LSC · 61525-62675 · 1151 bp

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Pi 0.0149

MarkerSeek score 58.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1151
variable_sites: 17
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCCATTACGTGTCCAAGGTC	ACTGGTATTCCTGTGATCGG	55.0	50.0	58.3	56.7	1338-1344 bp	1.0	69.1
petA-psbJ_p2	CCCATTACGTGTCCAAGGTC	CTGGTATTCCTGTGATCGGT	55.0	50.0	58.3	56.7	1337-1343 bp	1.0	69.1
petA-psbJ_p3	GACCCATTACGTGTCCAAGG	ACTGGTATTCCTGTGATCGG	55.0	50.0	58.3	56.7	1340-1346 bp	1.0	69.1
petA-psbJ_p4	GACCCATTACGTGTCCAAGG	CTGGTATTCCTGTGATCGGT	55.0	50.0	58.3	56.7	1339-1345 bp	1.0	69.1
petA-psbJ_p5	ACGTGTCCAAGGTCTTTTGT	CTGGTATTCCTGTGATCGGT	45.0	50.0	57.6	56.7	1331-1337 bp	1.0	67.9