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rbcL-accD

LSC · 55061-55824 · 764 bp

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Pi 0.00403

MarkerSeek score 63.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 3
pi: 0.00403
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	ACATCGAATTGAACCACCGT	55.0	45.0	58.0	57.8	863-882 bp	1.0	80.3
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	GCCGACACCTGGATTCTAAC	55.0	55.0	58.0	58.3	896-915 bp	1.0	78.9
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	TTTAGCCGACACCTGGATTC	55.0	50.0	58.0	57.6	900-919 bp	1.0	78.4
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	ACATCGAATTGAACCACCGT	55.0	45.0	57.7	57.8	849-868 bp	1.0	78.3
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	AGCCGACACCTGGATTCTAA	55.0	50.0	58.0	58.4	897-916 bp	1.0	78.2