Hotspot

trnM-CAU-atpE

LSC · 50733-51021 · 289 bp

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Pi 0.0285

MarkerSeek score 58.80

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 289
variable_sites: 8
pi: 0.0285
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GCGGGAGTCATTGGTTCAAA	ACGAGTAGAGGCTATCAATGTG	50.0	45.5	58.8	57.4	354-386 bp	1.0	56.8
trnM-CAU-atpE_p2	GCGGGAGTCATTGGTTCAAA	CGAGTAGAGGCTATCAATGTGA	50.0	45.5	58.8	57.2	353-385 bp	1.0	54.7
trnM-CAU-atpE_p3	AGAGTATCGCTTTCATACGGC	ACGAGTAGAGGCTATCAATGTG	47.6	45.5	58.0	57.4	373-405 bp	1.0	54.6
trnM-CAU-atpE_p4	CTTTCATACGGCGGGAGTC	ACGAGTAGAGGCTATCAATGTG	57.9	45.5	58.0	57.4	364-396 bp	1.0	54.5
trnM-CAU-atpE_p5	GCGGGAGTCATTGGTTCAA	ACGAGTAGAGGCTATCAATGTG	52.6	45.5	58.1	57.4	354-386 bp	1.0	54.4