Hotspot

trnD-GUC-trnY-GUA

LSC · 28780-29193 · 414 bp

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Pi 0.0122

MarkerSeek score 62.50

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 5
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTGAATTGGGATCCGTCGG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	540-545 bp	1.0	79.3
trnD-GUC-trnY-GUA_p2	ATTGAATTGGGATCCGTCGG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.0	57.8	539-544 bp	1.0	79.3
trnD-GUC-trnY-GUA_p3	ATTGAATTGGGATCCGTCGG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.0	58.4	561-566 bp	1.0	78.3
trnD-GUC-trnY-GUA_p4	ATTGAATTGGGATCCGTCGG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.0	57.6	560-565 bp	1.0	78.2
trnD-GUC-trnY-GUA_p5	ATTGAATTGGGATCCGTCGG	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.0	58.4	594-599 bp	1.0	77.8