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petN-psbM

LSC · 27475-27971 · 497 bp

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Pi 0.0086

MarkerSeek score 65.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 497
variable_sites: 4
pi: 0.0086
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCACTGTTCATTCTAGTCCC	45.0	47.6	58.0	57.7	615-646 bp	1.0	73.8
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	621-652 bp	1.0	73.2
petN-psbM_p3	AGTAAGTCTTGCTTGGGCTG	TGCACTGTTCATTCTAGTCCC	50.0	47.6	57.8	57.7	621-652 bp	1.0	72.3
petN-psbM_p4	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	627-658 bp	1.0	71.7
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	GCACTGTTCATTCTAGTCCCT	45.0	47.6	58.0	57.4	614-645 bp	1.0	71.5