Hotspot

atpA-atpF

LSC · 11471-11526 · 56 bp

Back to Leptopus

Pi 0.0566

MarkerSeek score 71.90

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 3
pi: 0.0566
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATGCCATCGCCTACTTGAAG	ATTGGCATATTTGGGGCGAT	50.0	45.0	58.0	58.0	208-211 bp	1.0	82.6
atpA-atpF_p2	CCCCGCCATTACTTCATCAA	ATTGGCATATTTGGGGCGAT	50.0	45.0	57.9	58.0	245-248 bp	1.0	82.1
atpA-atpF_p3	ATGCCATCGCCTACTTGAAG	GAAGCGGATCAGTTTCGAGT	50.0	50.0	58.0	58.0	454-457 bp	1.0	81.2
atpA-atpF_p4	CCCCGCCATTACTTCATCAA	GAAGCGGATCAGTTTCGAGT	50.0	50.0	57.9	58.0	491-494 bp	1.0	81.0
atpA-atpF_p5	AATTCCCCCGCCATTACTTC	GAAGCGGATCAGTTTCGAGT	50.0	50.0	57.9	58.0	496-499 bp	1.0	80.9