Hotspot

psbI-trnS-GCU

LSC · 7800-7936 · 137 bp

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Pi 0.0229

MarkerSeek score 69.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 3
pi: 0.0229
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGACGCGAAGAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.7	58.0	227-233 bp	1.0	80.8
psbI-trnS-GCU_p2	GTAATCCTGGACGCGAAGAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.7	58.0	228-234 bp	1.0	80.8
psbI-trnS-GCU_p3	GTAATCCTGGACGCGAAGAA	GACTAAAGCGTCGGATTGCT	50.0	50.0	57.7	58.4	225-231 bp	1.0	78.0
psbI-trnS-GCU_p4	GTAATCCTGGACGCGAAGAA	TTTGGGAGAGATGGCTGAGT	50.0	50.0	57.7	58.3	246-252 bp	1.0	77.9
psbI-trnS-GCU_p5	GTAATCCTGGACGCGAAGAA	CTGAGTGGACTAAAGCGTCG	50.0	55.0	57.7	58.4	232-238 bp	1.0	77.7