Hotspot

trnE-UUC-rrn16

IRa · 136357-136660 · 304 bp

Back to Leptodermis

Pi 0.00825

MarkerSeek score 52.90

Primer available yes

Label trnE-UUC-rrn16

Feature Metadata

label_name: trnE-UUC-rrn16
feature_type: igs
region: IRa
length_bp: 304
variable_sites: 11
pi: 0.00825
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rrn16_p1	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.2	57.7	386-398 bp	1.0	81.0
trnE-UUC-rrn16_p2	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	55.0	55.0	57.8	57.7	418-430 bp	1.0	80.8
trnE-UUC-rrn16_p3	GGCACAACGACGCAATTATC	AGTGACTGGAGTGAAGTCGT	50.0	50.0	58.2	58.3	408-420 bp	1.0	80.7
trnE-UUC-rrn16_p4	CTATCCATGTGGCTGAGAGC	AGTGACTGGAGTGAAGTCGT	55.0	50.0	57.8	58.3	440-452 bp	1.0	80.5
trnE-UUC-rrn16_p5	CTATCCATGTGGCTGAGAGC	AAGTCGTAACAAGGTAGCCG	55.0	50.0	57.8	57.6	427-439 bp	1.0	80.2