Hotspot

rpl32-trnL-UAG

SSC · 113736-114423 · 688 bp

Back to Leptodermis

Pi 0.0118

MarkerSeek score 48.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 688
variable_sites: 23
pi: 0.0118
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CCACGAGTTTGCATTTGGTC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1437-1450 bp	1.0	81.8
rpl32-trnL-UAG_p2	CCACGAGTTTGCATTTGGTC	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.9	57.8	1430-1443 bp	1.0	81.3
rpl32-trnL-UAG_p3	CCACGAGTTTGCATTTGGTC	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.9	57.8	1436-1449 bp	1.0	81.2
rpl32-trnL-UAG_p4	CCACGAGTTTGCATTTGGTC	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.9	57.8	1435-1448 bp	1.0	81.2
rpl32-trnL-UAG_p5	CCACGAGTTTGCATTTGGTC	TGTCCCGAATAAACCCAACG	50.0	50.0	57.9	58.2	1768-1781 bp	1.0	80.8