Hotspot

rrn16-trnE-UUC

IRb · 101615-101918 · 304 bp

Back to Leptodermis

Pi 0.00825

MarkerSeek score 52.90

Primer available yes

Label rrn16-trnE-UUC

Feature Metadata

label_name: rrn16-trnE-UUC
feature_type: igs
region: IRb
length_bp: 304
variable_sites: 11
pi: 0.00825
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnE-UUC_p1	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	386-398 bp	1.0	81.0
rrn16-trnE-UUC_p2	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	55.0	55.0	57.7	57.8	418-430 bp	1.0	80.8
rrn16-trnE-UUC_p3	AGTGACTGGAGTGAAGTCGT	GGCACAACGACGCAATTATC	50.0	50.0	58.3	58.2	408-420 bp	1.0	80.7
rrn16-trnE-UUC_p4	AGTGACTGGAGTGAAGTCGT	CTATCCATGTGGCTGAGAGC	50.0	55.0	58.3	57.8	440-452 bp	1.0	80.5
rrn16-trnE-UUC_p5	AAGTCGTAACAAGGTAGCCG	CTATCCATGTGGCTGAGAGC	50.0	55.0	57.6	57.8	427-439 bp	1.0	80.2