Hotspot

trnP-UGG-psaJ

LSC · 66394-66789 · 396 bp

Back to Leptodermis

Pi 0.00645

MarkerSeek score 52.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 396
variable_sites: 8
pi: 0.00645
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CTAAAGCCCCGAACCAAAGT	50.0	50.0	58.1	58.1	507-518 bp	1.0	81.4
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CTAAAGCCCCGAACCAAAGT	50.0	50.0	58.1	58.1	506-517 bp	1.0	81.4
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	CTAAAGCCCCGAACCAAAGT	50.0	50.0	58.1	58.1	518-529 bp	1.0	81.3
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	CTAAAGCCCCGAACCAAAGT	50.0	50.0	58.1	58.1	517-528 bp	1.0	81.3
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	CTAAAGCCCCGAACCAAAGT	50.0	50.0	58.1	58.1	520-531 bp	1.0	81.2