Hotspot

psbF-psbE

LSC · 64293-64301 · 9 bp

Back to Leptodermis

Pi 0.0617

MarkerSeek score 63.10

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 9
variable_sites: 1
pi: 0.0617
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	AGAAGACGGTAGGGATAGCC	ATGTGTTTGGAAGCCCTAGG	55.0	50.0	58.0	57.5	207 bp	1.0	76.3
psbF-psbE_p2	GATAGCCAGTCCGTGAACAG	ATGTGTTTGGAAGCCCTAGG	55.0	50.0	58.1	57.5	194 bp	1.0	75.7
psbF-psbE_p3	GAACAGCCAACCATCGTACT	ATGTGTTTGGAAGCCCTAGG	50.0	50.0	57.9	57.5	180 bp	1.0	75.6
psbF-psbE_p4	AACAGCCAACCATCGTACTG	ATGTGTTTGGAAGCCCTAGG	50.0	50.0	58.2	57.5	179 bp	1.0	74.8
psbF-psbE_p5	TGAACAGCCAACCATCGTAC	ATGTGTTTGGAAGCCCTAGG	50.0	50.0	58.2	57.5	181 bp	1.0	74.8