Hotspot

petN-psbM

LSC · 28853-29542 · 690 bp

Back to Leptodermis

Pi 0.00816
MarkerSeek score 42.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
690
variable_sites
17
pi
0.00816
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 CGCTTTGGCACAGAGATAGT CTATGAAAGGAAAGGGGGCC 50.0 55.0 58.0 57.9 1829-1861 bp 1.0 81.9
petN-psbM_p2 CGCTTTGGCACAGAGATAGT GGCTATGAAAGGAAAGGGGG 50.0 55.0 58.0 57.9 1831-1863 bp 1.0 81.8
petN-psbM_p3 CGCTTTGGCACAGAGATAGT GATTGGGTCCCGAATCGAAA 50.0 50.0 58.0 58.0 1495-1521 bp 1.0 81.5
petN-psbM_p4 CGCTTTGGCACAGAGATAGT TTTTTGGCAGGAAAAGGGGT 50.0 45.0 58.0 58.1 1751-1784 bp 1.0 81.1
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT GATTGGGTCCCGAATCGAAA 50.0 50.0 58.2 58.0 1084-1110 bp 1.0 80.6