Hotspot

rps2-rpoC2

LSC · 16370-16595 · 226 bp

Back to Leptodermis

Pi 0.0268

MarkerSeek score 52.40

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 226
variable_sites: 13
pi: 0.0268
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCTGCCTCCATCATCTCTTC	TCCATGATACATCAGAACAGTCT	55.0	39.1	57.5	56.8	321-326 bp	1.0	45.7
rps2-rpoC2_p2	ATGGCCAAAATGAACCCCTG	TCCATGATACATCAGAACAGTCT	50.0	39.1	58.7	56.8	337-342 bp	1.0	44.1
rps2-rpoC2_p3	CATGGCCAAAATGAACCCCT	TCCATGATACATCAGAACAGTCT	50.0	39.1	58.7	56.8	338-343 bp	1.0	44.1
rps2-rpoC2_p4	CCCTGCCTCCATCATCTCTT	TCCATGATACATCAGAACAGTCT	55.0	39.1	58.9	56.8	322-327 bp	1.0	43.7
rps2-rpoC2_p5	CCTGCCTCCATCATCTCTTC	TCCTTTCAAAGAGTTTCCATGA	55.0	36.4	57.5	55.6	336-341 bp	1.0	43.6