Hotspot

atpH-atpI

LSC · 13664-14682 · 1019 bp

Back to Leptodermis

Pi 0.00399

MarkerSeek score 56.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1019
variable_sites: 12
pi: 0.00399
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAAACCAGCAGCAATAACGG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.0	58.5	1120-1160 bp	1.0	76.8
atpH-atpI_p2	CCAAACCAGCAGCAATAACG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.0	58.5	1121-1161 bp	1.0	76.8
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1107-1147 bp	1.0	76.7
atpH-atpI_p4	TCCGATAGAAGCAAGCCCTA	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1144-1184 bp	1.0	76.3
atpH-atpI_p5	AACCAGCAGCAATAACGGAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	1118-1158 bp	1.0	76.2