Hotspot

trnS-GCU-trnG-GCC

LSC · 8532-9122 · 591 bp

Back to Leptodermis

Pi 0.012
MarkerSeek score 49.90
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
591
variable_sites
19
pi
0.012
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA AAACCGAAAGCAAAGACCCT 50.0 45.0 58.0 57.9 703-868 bp 1.0 82.9
trnS-GCU-trnG-GCC_p2 CAATCCGACGCTTTAGTCCA AACCGAAAGCAAAGACCCTT 50.0 45.0 58.0 57.9 702-867 bp 1.0 82.9
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA ACCGAAAGCAAAGACCCTTT 50.0 45.0 58.0 57.9 701-866 bp 1.0 82.9
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC AACCGAAAGCAAAGACCCTT 50.0 45.0 58.0 57.9 701-866 bp 1.0 82.9
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC ACCGAAAGCAAAGACCCTTT 50.0 45.0 58.0 57.9 700-865 bp 1.0 82.9