Hotspot

rps16-trnQ-UUG

LSC · 5777-7189 · 1413 bp

Back to Leptodermis

Pi 0.0102

MarkerSeek score 54.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1413
variable_sites: 40
pi: 0.0102
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1417-1486 bp	1.0	84.5
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1421-1490 bp	1.0	79.8
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1447-1516 bp	1.0	79.6
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1409-1478 bp	1.0	79.3
rps16-trnQ-UUG_p5	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.9	58.1	1416-1485 bp	1.0	78.7