Hotspot

rps3

LSC · 79038-79712 · 675 bp

Back to Leptochloa

Pi 0.00148

MarkerSeek score 59.60

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 675
variable_sites: 1
pi: 0.00148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	CACTGGCCAGAATGAGAAGA	ACCTAAGATTTCGATTCCCAGT	50.0	40.9	57.2	56.9	750 bp	1.0	49.0
rps3_p2	CTGGCCAGAATGAGAAGACA	ACCTAAGATTTCGATTCCCAGT	50.0	40.9	57.2	56.9	748 bp	1.0	49.0
rps3_p3	TCACTGGCCAGAATGAGAAG	ACCTAAGATTTCGATTCCCAGT	50.0	40.9	57.2	56.9	751 bp	1.0	49.0
rps3_p4	ACTGGCCAGAATGAGAAGAC	ACCTAAGATTTCGATTCCCAGT	50.0	40.9	57.2	56.9	749 bp	1.0	49.0
rps3_p5	TGGCCAGAATGAGAAGACAAG	ACCTAAGATTTCGATTCCCAGT	47.6	40.9	57.9	56.9	747 bp	1.0	46.7