Hotspot

rps2

LSC · 29873-30583 · 711 bp

Back to Leptochloa

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 1
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TCGGCTCTTTCTTAATCTTCA	TCCCATAAATCTACGTAGTTGCT	38.1	39.1	54.7	57.1	847 bp	1.0	40.1
rps2_p2	TCGGCTCTTTCTTAATCTTCAA	TCCCATAAATCTACGTAGTTGCT	36.4	39.1	55.5	57.1	847 bp	1.0	40.1
rps2_p3	TTCGGCTCTTTCTTAATCTTCA	TCCCATAAATCTACGTAGTTGCT	36.4	39.1	55.5	57.1	848 bp	1.0	40.1
rps2_p4	TCGGCTCTTTCTTAATCTTCA	TCCCATAAATCTACGTAGTTGCTT	38.1	37.5	54.7	57.8	847 bp	1.0	40.1
rps2_p5	TCGGCTCTTTCTTAATCTTCA	TCCCATAAATCTACGTAGTTGC	38.1	40.9	54.7	55.7	847 bp	1.0	40.1