Hotspot

rps16

LSC · 4493-5544 · 1052 bp

Back to Leptochloa

Pi 0

MarkerSeek score 58.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1052
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GCACGACATAAATGCAGCAA	ACTCTCGATACCACTTTGCA	45.0	45.0	57.7	56.6	1208-1209 bp	1.0	66.6
rps16_p2	GCACGACATAAATGCAGCAA	AACTCTCGATACCACTTTGCA	45.0	42.9	57.7	57.3	1209-1210 bp	1.0	64.6
rps16_p3	GCACGACATAAATGCAGCAA	ACTCTCGATACCACTTTGCAA	45.0	42.9	57.7	57.3	1208-1209 bp	1.0	64.6
rps16_p4	GCACGACATAAATGCAGCAA	CAACTCTCGATACCACTTTGC	45.0	47.6	57.7	57.0	1210-1211 bp	1.0	62.0
rps16_p5	GCACGACATAAATGCAGCAA	AACTCTCGATACCACTTTGCAA	45.0	40.9	57.7	58.0	1209-1210 bp	1.0	62.0