Hotspot

rpl16

LSC · 77465-78895 · 1431 bp

Back to Leptochloa

Pi 0.000699

MarkerSeek score 59.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1431
variable_sites: 1
pi: 0.000699
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CTCCAGAAGGAAAACCTGGG	TTTCCAAAAATCCCAGAAAAA	55.0	28.6	57.8	52.2	1571-1572 bp	1.0	40.1
rpl16_p2	CTCCAGAAGGAAAACCTGGG	TTTTCCAAAAATCCCAGAAAA	55.0	28.6	57.8	52.2	1572-1573 bp	1.0	40.1
rpl16_p3	CTCCAGAAGGAAAACCTGGG	TTTTTCCAAAAATCCCAGAAA	55.0	28.6	57.8	52.2	1573-1574 bp	1.0	40.1
rpl16_p4	CTCCAGAAGGAAAACCTGGG	TTTTCCAAAAATCCCAGAAAAA	55.0	27.3	57.8	53.1	1572-1573 bp	1.0	40.1
rpl16_p5	CTCCAGAAGGAAAACCTGGG	TTTTTCCAAAAATCCCAGAAAA	55.0	27.3	57.8	53.1	1573-1574 bp	1.0	40.1