Hotspot

matK

LSC · 1692-3233 · 1542 bp

Back to Leptochloa

Pi 0.000649
MarkerSeek score 59.00
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1542
variable_sites
1
pi
0.000649
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 AGGGGAACATTTCAGAATGGA TGTAACACCCTGTTTTGACCA 42.9 42.9 56.9 57.6 1713 bp 1.0 52.2
matK_p2 AGGGGAACATTTCAGAATGGAA TGTAACACCCTGTTTTGACCA 40.9 42.9 57.6 57.6 1713 bp 1.0 49.9
matK_p3 GGGGAACATTTCAGAATGGA TGTAACACCCTGTTTTGACCA 45.0 42.9 55.3 57.6 1712 bp 1.0 47.4
matK_p4 AGGGGAACATTTCAGAATGG TGTAACACCCTGTTTTGACCA 45.0 42.9 55.3 57.6 1713 bp 1.0 47.3
matK_p5 AGGGGAACATTTCAGAATGGA TGTAACACCCTGTTTTGACCAT 42.9 40.9 56.9 58.1 1713 bp 1.0 46.3