Hotspot

rpl33-rps18

LSC · 65189-65491 · 303 bp

Back to Leptochloa

Pi 0.00372

MarkerSeek score 57.70

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 303
variable_sites: 1
pi: 0.00372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGAATCGCCACAATACACCC	ACGAAAAGGTTGCTTGGATTTT	50.0	36.4	58.0	57.6	451-485 bp	1.0	64.7
rpl33-rps18_p2	CCACAATACACCCGGACAAT	ACGAAAAGGTTGCTTGGATTTT	50.0	36.4	57.9	57.6	444-478 bp	1.0	64.0
rpl33-rps18_p3	CCGCAAGCATACGACTCATA	ACGAAAAGGTTGCTTGGATTTT	50.0	36.4	57.9	57.6	396-430 bp	1.0	63.9
rpl33-rps18_p4	AGAATCGCCACAATACACCC	TTACGAAAAGGTTGCTTGGA	50.0	40.0	58.0	55.5	453-487 bp	1.0	63.9
rpl33-rps18_p5	CCACAATACACCCGGACAAT	TTACGAAAAGGTTGCTTGGA	50.0	40.0	57.9	55.5	446-480 bp	1.0	63.2