Hotspot

ndhC-trnV-UAC

LSC · 50146-51067 · 922 bp

Back to Leptochloa

Pi 0.00109

MarkerSeek score 59.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 922
variable_sites: 1
pi: 0.00109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGGTCCTTTATTAATCGGGGC	GGTTCGAATCCGTATAGCCC	47.6	55.0	57.8	57.9	1057-1058 bp	1.0	69.8
ndhC-trnV-UAC_p2	TGGTCCTTTATTAATCGGGGC	AGCCCAATGCAGAAAAATGC	47.6	45.0	57.8	57.9	1150-1151 bp	1.0	69.6
ndhC-trnV-UAC_p3	TGGTCCTTTATTAATCGGGGC	CGGTTCGAATCCGTATAGCC	47.6	55.0	57.8	58.3	1058-1059 bp	1.0	68.0
ndhC-trnV-UAC_p4	GGTCCTTTATTAATCGGGGCT	GGTTCGAATCCGTATAGCCC	47.6	55.0	57.5	57.9	1056-1057 bp	1.0	67.5
ndhC-trnV-UAC_p5	GGTCCTTTATTAATCGGGGCT	AGCCCAATGCAGAAAAATGC	47.6	45.0	57.5	57.9	1149-1150 bp	1.0	67.3