Hotspot

psbK-psbI

LSC · 7383-7776 · 394 bp

Back to Leptochloa

Pi 0.00254

MarkerSeek score 55.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 394
variable_sites: 1
pi: 0.00254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	TTTACTCCTCACGTCCAGGA	50.0	50.0	58.0	57.7	585 bp	1.0	77.6
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTTACTCCTCACGTCCAGGA	50.0	50.0	57.9	57.7	536 bp	1.0	77.3
psbK-psbI_p3	TTAGCGTTTGTTTGGCAAGC	TTTACTCCTCACGTCCAGGA	45.0	50.0	58.2	57.7	545 bp	1.0	76.5
psbK-psbI_p4	CGTGGATGTTATGCCTGTCA	ACTCCTCACGTCCAGGATTA	50.0	50.0	58.0	57.5	582 bp	1.0	75.7
psbK-psbI_p5	CGTGGATGTTATGCCTGTCA	TACTCCTCACGTCCAGGATT	50.0	50.0	58.0	57.5	583 bp	1.0	75.7