Hotspot

trnL-UAG-ccsA

Genome · 110417-110767 · 351 bp

Back to Leptochilus

Pi 0.0661

MarkerSeek score 79.80

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: Genome
length_bp: 351
variable_sites: 22
pi: 0.0661
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	CTCTTAGGAAGCAGTGCCAG	GGATAGAAGCGCGTTCCATA	55.0	50.0	58.0	57.6	415-433 bp	1.0	83.9
trnL-UAG-ccsA_p2	CTCTTAGGAAGCAGTGCCAG	TATGGATAGAAGCGCGTTCC	55.0	50.0	58.0	57.6	418-436 bp	1.0	83.9
trnL-UAG-ccsA_p3	CTTAGGAAGCAGTGCCAGAG	GGATAGAAGCGCGTTCCATA	55.0	50.0	58.0	57.6	413-431 bp	1.0	83.9
trnL-UAG-ccsA_p4	CTTAGGAAGCAGTGCCAGAG	TATGGATAGAAGCGCGTTCC	55.0	50.0	58.0	57.6	416-434 bp	1.0	83.9
trnL-UAG-ccsA_p5	ATAGGTAGACACGCTGCTCT	GGATAGAAGCGCGTTCCATA	50.0	50.0	57.9	57.6	431-449 bp	1.0	83.3