Hotspot

rpl32-trnP-GGG

Genome · 109345-110026 · 682 bp

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Pi 0.0784
MarkerSeek score 81.00
Primer available yes
Label rpl32-trnP-GGG

Feature Metadata

label_name
rpl32-trnP-GGG
feature_type
igs
region
Genome
length_bp
682
variable_sites
48
pi
0.0784
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnP-GGG_p1 TGACGGAAGAGAGAGACTCC GTGATGGAGGTCACAGGTTC 55.0 55.0 57.9 57.9 670-751 bp 1.0 87.7
rpl32-trnP-GGG_p2 TGACGGAAGAGAGAGACTCC TGATGGAGGTCACAGGTTCA 55.0 50.0 57.9 58.3 669-750 bp 1.0 86.3
rpl32-trnP-GGG_p3 ACGGAAGAGAGAGACTCCTC GTGATGGAGGTCACAGGTTC 55.0 55.0 57.6 57.9 668-749 bp 1.0 85.3
rpl32-trnP-GGG_p4 GACGGAAGAGAGAGACTCCT GTGATGGAGGTCACAGGTTC 55.0 55.0 57.6 57.9 669-750 bp 1.0 85.3
rpl32-trnP-GGG_p5 ACGGAAGAGAGAGACTCCTC TGATGGAGGTCACAGGTTCA 55.0 50.0 57.6 58.3 667-748 bp 1.0 83.9