Hotspot

rpl2-rpl23

Genome · 83745-83813 · 69 bp

Back to Leptochilus

Pi 0.116

MarkerSeek score 73.60

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: Genome
length_bp: 69
variable_sites: 8
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	ATTGCTGCGACCACTTCTAG	TGCGAGGCAGGTATATCTGA	50.0	50.0	58.0	58.0	591 bp	1.0	83.1
rpl2-rpl23_p2	CTGGCTACTCTTCCGTCAAC	TGCGAGGCAGGTATATCTGA	55.0	50.0	58.0	58.0	695 bp	1.0	82.8
rpl2-rpl23_p3	CTGGCTACTCTTCCGTCAAC	AGTCTATTCGCCTGTTGCAG	55.0	50.0	58.0	58.0	541 bp	1.0	82.7
rpl2-rpl23_p4	CTGGCTACTCTTCCGTCAAC	GAGGAGAGGATCGATGGGAA	55.0	55.0	58.0	58.0	628 bp	0.5	60.0
rpl2-rpl23_p5	ATTGCTGCGACCACTTCTAG	GAGGAGAGGATCGATGGGAA	50.0	55.0	58.0	58.0	524 bp	0.5	59.9