Hotspot

psaI-ycf4

Genome · 57960-58391 · 432 bp

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Pi 0.0425

MarkerSeek score 77.80

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: Genome
length_bp: 432
variable_sites: 18
pi: 0.0425
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	CGTACCCCTAGTTGGTTTGG	CACCGCAGACAAGGATACAA	55.0	50.0	57.9	57.9	600-608 bp	1.0	81.9
psaI-ycf4_p2	CGTACCCCTAGTTGGTTTGG	AATTCGCAACTGTACGGGAA	55.0	45.0	57.9	57.8	570-578 bp	1.0	81.4
psaI-ycf4_p3	CGTACCCCTAGTTGGTTTGG	GAATTCGCAACTGTACGGGA	55.0	50.0	57.9	58.3	571-579 bp	1.0	80.5
psaI-ycf4_p4	CGTACCCCTAGTTGGTTTGG	CAAGCCCAACATGAATTCGC	55.0	50.0	57.9	58.4	583-591 bp	1.0	79.8
psaI-ycf4_p5	CCCCTAGTTGGTTTGGTGTT	CACCGCAGACAAGGATACAA	50.0	50.0	57.6	57.9	596-604 bp	1.0	79.7