Hotspot

rps4-trnL-UAA

Genome · 46796-47425 · 630 bp

Back to Leptochilus

Pi 0.0397

MarkerSeek score 71.90

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: Genome
length_bp: 630
variable_sites: 25
pi: 0.0397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	TCAGTTTCAAACGAGGTCCTC	CTCCACATCCAGGCTCAATC	47.6	55.0	57.9	58.0	723 bp	1.0	73.6
rps4-trnL-UAA_p2	TCAGTTTCAAACGAGGTCCTC	GCCCGATAAATGATCCCAGG	47.6	55.0	57.9	58.2	777 bp	1.0	72.3
rps4-trnL-UAA_p3	TCAGTTTCAAACGAGGTCCTC	GGGCTACCACTTGATACGTC	47.6	55.0	57.9	57.8	742 bp	1.0	72.1
rps4-trnL-UAA_p4	TCAGTTTCAAACGAGGTCCT	CTCCACATCCAGGCTCAATC	45.0	55.0	56.7	58.0	723 bp	1.0	72.1
rps4-trnL-UAA_p5	TCAGTTTCAAACGAGGTCCTC	CCACATCCAGGCTCAATCTG	47.6	55.0	57.9	58.3	721 bp	1.0	71.3