Hotspot

atpA-atpF

Genome · 13392-13477 · 86 bp

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Pi 0.0581

MarkerSeek score 71.50

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: Genome
length_bp: 86
variable_sites: 5
pi: 0.0581
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	TTTGCTTGCGAATGATGCTG	GAGCCATGGATAGCAAGTCC	45.0	55.0	58.0	58.1	164 bp	1.0	81.4
atpA-atpF_p2	TTTGCTTGCGAATGATGCTG	CCATGGATAGCAAGTCCGAC	45.0	55.0	58.0	58.1	161 bp	1.0	81.3
atpA-atpF_p3	TTTGCTTGCGAATGATGCTG	TCAACTGCACTTGCGAATGA	45.0	45.0	58.0	58.4	208 bp	1.0	78.5
atpA-atpF_p4	TGCTGCTAATTTCGTCAGGT	GAGCCATGGATAGCAAGTCC	45.0	55.0	57.5	58.1	149 bp	1.0	78.0
atpA-atpF_p5	TGCTGCTAATTTCGTCAGGT	CCATGGATAGCAAGTCCGAC	45.0	55.0	57.5	58.1	146 bp	1.0	77.9