Hotspot

trnL(UAA)

LSC · 48835-48884 · 50 bp

Back to Leptaspis

Pi 0.02

MarkerSeek score 64.00

Primer available yes

Label trnL(UAA)

Feature Metadata

label_name: trnL(UAA)
feature_type: tRNA
region: LSC
length_bp: 50
variable_sites: 1
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL(UAA)_p1	CGAGGATAAAGAGAGAGTCCC	CTCTTCGGACTCCTTTGTGA	52.4	50.0	56.7	56.9	233-239 bp	1.0	53.3
trnL(UAA)_p2	CGAGGATAAAGAGAGAGTCCC	TCTCTTCGGACTCCTTTGTG	52.4	50.0	56.7	56.9	234-240 bp	1.0	53.3
trnL(UAA)_p3	ACGAGGATAAAGAGAGAGTCCC	CTCTTCGGACTCCTTTGTGA	50.0	50.0	58.4	56.9	234-240 bp	1.0	51.9
trnL(UAA)_p4	ACGAGGATAAAGAGAGAGTCCC	TCTCTTCGGACTCCTTTGTG	50.0	50.0	58.4	56.9	235-241 bp	1.0	51.9
trnL(UAA)_p5	CGAGGATAAAGAGAGAGTCCCA	CTCTTCGGACTCCTTTGTGA	50.0	50.0	58.4	56.9	233-239 bp	1.0	51.9