Hotspot

rpoC2

LSC · 26810-31291 · 4482 bp

Back to Leptaspis

Pi 0.00764

MarkerSeek score 44.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4482
variable_sites: 34
pi: 0.00764
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GTTGGCGAAAAAGCGAAGAA	CAAGAGATCCTGCGGGAAAA	45.0	50.0	57.9	57.9	1045-1060 bp	1.0	82.3
rpoC2_p2	TTCGCTCCACAAGGATCAAG	CAAGAGATCCTGCGGGAAAA	50.0	50.0	57.9	57.9	1270-1285 bp	1.0	81.7
rpoC2_p3	TTCGCTCCACAAGGATCAAG	TTTCCTGCAAGAGATCCTGC	50.0	50.0	57.9	57.9	1277-1292 bp	1.0	81.7
rpoC2_p4	AAAGAGAAAAAGGGCTCGGG	CAAGAGATCCTGCGGGAAAA	50.0	50.0	58.1	57.9	882 bp	0.5	58.4
rpoC2_p5	AAAGAGAAAAAGGGCTCGGG	TTTCCTGCAAGAGATCCTGC	50.0	50.0	58.1	57.9	889 bp	0.5	58.3