Hotspot

ndhF-rpl32

SSC · 108383-109289 · 907 bp

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Pi 0.015
MarkerSeek score 62.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
907
variable_sites
13
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGGAGAAAAGGT TCCAAAGATTTTTACGAATACGCT 50.0 33.3 57.0 57.3 977-1020 bp 1.0 44.5
ndhF-rpl32_p2 CTGGAAGTGGGAGAAAAGGT TCCAAAGATTTTTACGAATACGC 50.0 34.8 57.0 55.9 977-1020 bp 1.0 44.5
ndhF-rpl32_p3 CTGGAAGTGGGAGAAAAGGT CCAAAGATTTTTACGAATACGCT 50.0 34.8 57.0 55.9 976-1019 bp 1.0 44.5
ndhF-rpl32_p4 CTGGAAGTGGGAGAAAAGGT TCCAAAGATTTTTACGAATACGCTT 50.0 32.0 57.0 57.9 977-1020 bp 1.0 44.5
ndhF-rpl32_p5 CTGGAAGTGGGAGAAAAGGT TTCCAAAGATTTTTACGAATACGCT 50.0 32.0 57.0 57.9 978-1021 bp 1.0 44.5