Hotspot

trnN(GUU)-rps15

IRb · 103993-105524 · 1532 bp

Back to Leptaspis

Pi 0.00271

MarkerSeek score 63.80

Primer available yes

Label trnN(GUU)-rps15

Feature Metadata

label_name: trnN(GUU)-rps15
feature_type: igs
region: IRb
length_bp: 1532
variable_sites: 4
pi: 0.00271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN(GUU)-rps15_p1	ACGACCAGTCAGTTAACAGC	CAGAACCCCTGTTTTCTTCT	50.0	45.0	57.8	55.2	1598-1654 bp	1.0	59.1
trnN(GUU)-rps15_p2	AGGATTCGAACCTACGACCA	CAGAACCCCTGTTTTCTTCT	50.0	45.0	58.2	55.2	1611-1667 bp	1.0	59.0
trnN(GUU)-rps15_p3	GGATTCGAACCTACGACCAG	CAGAACCCCTGTTTTCTTCT	55.0	45.0	57.8	55.2	1610-1666 bp	1.0	58.6
trnN(GUU)-rps15_p4	ACGACCAGTCAGTTAACAGC	ACTTGAAATTCAACAGAACCCC	50.0	40.9	57.8	57.1	1611-1667 bp	1.0	58.2
trnN(GUU)-rps15_p5	AGGATTCGAACCTACGACCA	ACTTGAAATTCAACAGAACCCC	50.0	40.9	58.2	57.1	1624-1680 bp	1.0	58.2