Hotspot

clpP-psbB

LSC · 70742-71307 · 566 bp

Back to Leptaspis

Pi 0.0061

MarkerSeek score 64.00

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 3
pi: 0.0061
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCCAAGTCGCTTCTTCATC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.9	57.9	656-730 bp	1.0	82.6
clpP-psbB_p2	TCAACCCAAGTCGCTTCTTC	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.1	57.9	659-733 bp	1.0	82.5
clpP-psbB_p3	ACCCAAGTCGCTTCTTCATC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	574-648 bp	1.0	81.7
clpP-psbB_p4	TCAACCCAAGTCGCTTCTTC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.1	58.2	577-651 bp	1.0	81.5
clpP-psbB_p5	ACCCAAGTCGCTTCTTCATC	TGCACCGAAAGTAATCGACC	50.0	50.0	57.9	58.3	622-696 bp	1.0	81.3