Hotspot

ycf4-cemA

LSC · 60618-61016 · 399 bp

Back to Leptaspis

Pi 0.0459

MarkerSeek score 73.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 18
pi: 0.0459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	CACCAGGGCAAAAGTACGAT	45.0	50.0	57.9	58.2	490-498 bp	1.0	82.0
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	CACCAGGGCAAAAGTACGAT	50.0	50.0	58.1	58.2	492-500 bp	1.0	81.7
ycf4-cemA_p3	ACAAAAAGCTGCCGAATTGG	CACCAGGGCAAAAGTACGAT	45.0	50.0	57.8	58.2	519-527 bp	1.0	81.2
ycf4-cemA_p4	TGCGCGTACCAATTGAAGTA	AGAGAAAGAGACCCACCAGG	45.0	55.0	57.9	58.3	503-511 bp	1.0	80.5
ycf4-cemA_p5	CTTGCGCGTACCAATTGAAG	AGAGAAAGAGACCCACCAGG	50.0	55.0	58.1	58.3	505-513 bp	1.0	80.2