Hotspot

psbK-psbI

LSC · 7900-8328 · 429 bp

Back to Leptaspis

Pi 0.021

MarkerSeek score 66.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 429
variable_sites: 9
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	TTTTTACTCCTCACGCCCAG	50.0	50.0	58.0	57.8	622-627 bp	1.0	79.5
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCACGCCCAG	50.0	50.0	57.9	57.8	573-578 bp	1.0	79.4
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	570-575 bp	1.0	77.4
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	571-576 bp	1.0	77.4
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	572-577 bp	1.0	77.4