Hotspot

rps16-trnQ(UUG)

LSC · 5728-7278 · 1551 bp

Back to Leptaspis

Pi 0.0106

MarkerSeek score 61.20

Primer available yes

Label rps16-trnQ(UUG)

Feature Metadata

label_name: rps16-trnQ(UUG)
feature_type: igs
region: LSC
length_bp: 1551
variable_sites: 11
pi: 0.0106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ(UUG)_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1110-1624 bp	1.0	82.9
rps16-trnQ(UUG)_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1121-1635 bp	1.0	82.4
rps16-trnQ(UUG)_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1114-1628 bp	1.0	81.8
rps16-trnQ(UUG)_p4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	1125-1639 bp	1.0	81.3
rps16-trnQ(UUG)_p5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	45.0	50.0	58.1	57.6	1116-1630 bp	1.0	80.1