Hotspot

trnS(UGA)-EI144_pgp28

LSC · 43184-43595 · 412 bp

Back to Lepocinclis

Pi 0.43
MarkerSeek score 77.90
Primer available yes
Label trnS(UGA)-EI144_pgp28

Feature Metadata

label_name
trnS(UGA)-EI144_pgp28
feature_type
igs
region
LSC
length_bp
412
variable_sites
266
pi
0.43
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS(UGA)-EI144_pgp28_p1 GGATGTAGCGCAGTTTGGTA TGTTTGGGAAGGTCAGTGTT 50.0 45.0 58.0 57.2 1054 bp 0.25 43.3
trnS(UGA)-EI144_pgp28_p2 CAAGGGTCACAGGTTCGAAT TGTTTGGGAAGGTCAGTGTT 50.0 45.0 57.8 57.2 1015 bp 0.25 42.0
trnS(UGA)-EI144_pgp28_p3 CCGAAACTTGGAGAGGTGTC TGTTTGGGAAGGTCAGTGTT 55.0 45.0 58.2 57.2 986 bp 0.25 41.8
trnS(UGA)-EI144_pgp28_p4 GGATGTAGCGCAGTTTGGTA ATGTTTGGGAAGGTCAGTGT 50.0 45.0 58.0 56.9 1055 bp 0.25 41.4
trnS(UGA)-EI144_pgp28_p5 GGATGTAGCGCAGTTTGGTA TGTTTGGGAAGGTCAGTGTTT 50.0 42.9 58.0 57.9 1054 bp 0.25 41.1