Hotspot

EI144_pgp58

LSC · 10734-12095 · 1362 bp

Back to Lepocinclis

Pi 0.397

MarkerSeek score 83.40

Primer available yes

Label EI144_pgp58

Feature Metadata

label_name: EI144_pgp58
feature_type: gene
region: LSC
length_bp: 1362
variable_sites: 845
pi: 0.397
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EI144_pgp58_p1	GGTGGTCGTGATCAAGAGTC	CCAACCATCACCACCAAAGA	55.0	50.0	58.0	58.0	2189-2791 bp	0.5	69.3
EI144_pgp58_p2	GGTGGTCGTGATCAAGAGTC	TCCAACCATCACCACCAAAG	55.0	50.0	58.0	58.0	2190-2792 bp	0.5	69.3
EI144_pgp58_p3	CAACTGGTTTTGCTTGGTGG	CCAACCATCACCACCAAAGA	50.0	50.0	58.1	58.0	2170-2772 bp	0.5	68.9
EI144_pgp58_p4	CAACTGGTTTTGCTTGGTGG	TCCAACCATCACCACCAAAG	50.0	50.0	58.1	58.0	2171-2773 bp	0.5	68.9
EI144_pgp58_p5	GGTGGTCGTGATCAAGAGTC	CGATGGATTCAAAGTCGGGT	55.0	50.0	58.0	58.0	2141 bp	0.25	49.8