Hotspot

EI144_pgp51

LSC · 16681-17710 · 1030 bp

Back to Lepocinclis

Pi 0.34

MarkerSeek score 83.30

Primer available yes

Label EI144_pgp51

Feature Metadata

label_name: EI144_pgp51
feature_type: gene
region: LSC
length_bp: 1030
variable_sites: 552
pi: 0.34
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EI144_pgp51_p1	CGGAGTAGAGCAGTCTGGTA	ACTGTAGGAGCAGGAGTTGT	55.0	50.0	58.0	58.0	1639-1671 bp	0.5	71.0
EI144_pgp51_p2	GGTTAATGTCGTGGGTTCGA	ACTGTAGGAGCAGGAGTTGT	50.0	50.0	57.9	58.0	1688-1715 bp	0.5	70.9
EI144_pgp51_p3	AAAGCGGATGTAGCTCAGTG	ACTGTAGGAGCAGGAGTTGT	50.0	50.0	58.0	58.0	1728 bp	0.25	49.7
EI144_pgp51_p4	GAATCCCATCATCCGCTTGT	ACTGTAGGAGCAGGAGTTGT	50.0	50.0	58.0	58.0	1670 bp	0.25	49.6
EI144_pgp51_p5	AGGCTTAAAGCGGATGTAGC	ACTGTAGGAGCAGGAGTTGT	50.0	50.0	58.0	58.0	1734 bp	0.25	49.5